Journal: bioRxiv

Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection

doi: 10.1101/449124

Figure Lengend Snippet: ( A ) Female and male mice were infected with 10 7 CFU of UPEC strain UTI89-RFP-kan R and bladders analyzed by flow cytometry at 24 hours PI. Graphs show ( A ) the number of CD3 + CD4 + T cells, CD3 − NK1.1 + NK cells, γδ T cells, and LTi-like cells (CD90 + CD25 + CD4 + CD3 − NK1.1 − MHC II − CD11b − ) in bladders. ( B ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show the number of CD3 + CD4 + T cells, CD3 − NK1.1 + NK cells, γδ T cells, and CD90 + CD25 + CD4 + CD3 − NK1.1 − MHC II − CD11b − LTi-like cells in bladders. ( C-E ) Graphs show the number of infected mice, determined by urine sampling, over time in ( C ) wildtype or RAG2 − /-γc mice, ( D ) mice treated with isotype or αNK1.1-depleting antibody, ( E ) mice treated with α-IL-17 neutralizing antibody. ( A-B ) Data are pooled from 2-3 experiments, n=4-5/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ( C-E ) Graphs are representative experiments, n=6-12 mice per experiment, at least 2 experiments each. * p<0.05, ** p<0.01, Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.

Article Snippet: For IL-17 neutralization, 83.3-111 µg/mouse α-IL-17 (clone 50104, BioTechne/R&D Systems) or 83.3-111 µg/mouse rat IgG2a isotype control (clone 54447, BioXcell) were delivered intravenously in 100 µL PBS.

Techniques: Infection, Flow Cytometry, Sampling, MANN-WHITNEY

Journal: Natural Product Communications

Article Title: The Effects of Resveratrol Targeting MicroRNA-4325P/PDGF-B to Regulate Tumor Angiogenesis in Osteosarcoma Microenvironment

doi: 10.1177/1934578x231156705

Figure Lengend Snippet: Figure 10. Angiogenesis is promoted in the sprouting phase of CAM by miR-432-5p/PDGFB signaling. After exposure to MG-63 cells or HOS cells containing coverslip, 6-day-old embryos’ CAM was excised and subjected to RT-PCR analysis. TEM8, CALD1, CXCR4, Tie-2 and Ephrin-B2 have been identified as angiogenesis-associated genes. The expression of β-actin mRNA was used to normalize the expression of these mRNAs. The results are expressed as the mean ± SD. *P < 0.05 & **P < 0.01 represent a significant decrease when compared to the control or inhibitor. One-way ANOVA followed by Tukey’s multiple comparison was used to analyze the data.

Article Snippet: Twenty microgram of isolated protein was then utilized or Western blot analyzed utilizing antibodies against PDGFB (1:1200, MAB2201, Biotechne, R&D systems, USA), Tie-2 (1:800; Biotechne R&D Systems, USA), Cycline A (1: 1000; Biotechne R&D Systems), Cdk1 (1:1000, Sigma Aldrich, USA), Cald1 (1:1000; Sigma Aldrich, USA), caspase-3 (Cell Signaling Technology, USA), Ephrin-B2 (1:800; Biotechne R&D systems, USA), CXCR4 (1:800; FAB170P, Biotechne R&D systems, USA), and α-tubulin (1:1200; Sigma Aldrich, USA) and CDK-2 (1:1200, Santa Cruz Biotechnology, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Comparison

Journal: Research in Pharmaceutical Sciences

Article Title: Naringenin potentiated β-sitosterol healing effect on the scratch wound assay

doi: 10.4103/1735-5362.272565

Figure Lengend Snippet: Percentage closure in scratch wound assay. Data are presented as mean ± SD; * P ≤ 0.05, ** P ≤ 0.01, and ** P ≤ 0.001 indicate significant differences with positive control. All treatments were significantly ( P ≤ 0.001) different from negative control. Platelet-derived growth factor was used as a positive control.

Article Snippet: PDGF was obtained from Biotechne-R&D, USA, and resveratrol was obtained from Tocris, USA.

Techniques: Scratch Wound Assay Assay, Positive Control, Negative Control, Control